NGS Data Formats and Analyses

Here are my slides from a session on NGS data formats and analyses that I gave as part of the EPIZONE Workshop on Next Generation Sequencing applications and Bioinformatics in Brussels in April 2016. It covers file formats such as FASTA, FASTQ, SAM, BAM, and VCF, and also goes over IUAPAC nucleotide ambiguity codes, read names, quality […]

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Bioinformatics tools to analyse viral genomics data

  We have recently written a review article entitled “Bioinformatics tools to analyse viral genomics data” for the OIE. In the review, we were unable to provide direct hyperlinks and references to all available tools, simply because there are too many, so we included them here. These commonly used bioinformatics tools are split into the following categories: […]

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Case studies of HTS applications

Here are my slides from the BBSRC WestBio DTP skills training session at University of Glasgow, Friday 26th June 2015. The talk was entitled “Case studies of HTS applications” and presents a number of case studies on the application on high-throughput sequencing (HTS), also known as next generation sequencing (NGS), to biological problems ranging from human […]

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A simple method to distinguish low frequency variants from Illumina sequence errors

RNA viruses have high mutation rates and exist within their hosts as large, complex and heterogeneous populations, comprising a spectrum of related but non-identical genome sequences. Next generation sequencing has revolutionised the study of viral populations by enabling the ultra deep sequencing of their genomes, and the subsequent identification of the full spectrum of variants […]

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Calculating dNdS for NGS datasets

vNvS Our upcoming tool vNvS calculates the dN/dS ratio at each site, codon and also for the sample as a whole, here is an explanation of the theory behind it. vNvS is currently in development – for more information email Richard.Orton@glasgow.ac.uk dN/dS dN/dS is the ratio of the number of nonsynonymous substitutions per non-synonymous site (pN) […]

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