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  • Tag: bcl2fastq

How to demultiplex Illumina data and generate fastq files using bcl2fastq

  • April 25, 2016
  • 4 Comments

Sequence runs on NGS instruments are typically carried out with multiple samples pooled together. An index tag (also called a barcode) consisting of a unique sequence of between 6 and 12bp is added to each sample so that the sequence reads from different samples can be identified. On the Illumina MiSeq, the process of demultiplexing […]

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