If you are using BaseSpace for sample entry but demultiplexing your data manually, you may have been frustrated that there is no facility to download your sample names and index tag data from BaseSpace as a sample sheet. This means you have to enter the same data twice – with the possibility of errors creeping […]
Read MoreHow to demultiplex Illumina data and generate fastq files using bcl2fastq
- April 25, 2016
- 4 Comments
Sequence runs on NGS instruments are typically carried out with multiple samples pooled together. An index tag (also called a barcode) consisting of a unique sequence of between 6 and 12bp is added to each sample so that the sequence reads from different samples can be identified. On the Illumina MiSeq, the process of demultiplexing […]
Read More